Patent No.: US 10m 130, 701 B2
Date of Patent: Nov. 20, 2018
The present invention provides a live, attenuated coronavirus comprising a variant replicase gene encoding polyproteins comprising a mutation in or more of non-structural proteins(s) (nsp)-10, nsp-15, or nsp-16. The coronavirus may be used as a vaccine for treating and/or preventing a disease, such as infectious bronchitis, in a subject.